Formal Salutation, e.g., "Dear Dr. John Smith:"
Formal Closing Words, e.g., "Sincerely yours," and "Best regards,"
Address the Recipient Formally
Brief Self-Introduction at the Beginning
Signature Including Title, Affiliation, Mailing Address, Phone Number, URL
End a Message with Thanks to a Recipient
e.g., "Thank you for your consideration on this matter." in case of a request email