Dive into the world of CRISPR in our exclusive 5-day lab workshop. From designing gRNAs and CRISPR constructs to advanced sequence analysis, explore the cutting-edge techniques revolutionizing genetic engineering. Don’t miss this incredible opportunity—register now for your free spot!
A free workshop for University of Hawai’i Manoa students
Dates: July 8 – 12, 2024
Hours: 1:00 – 6:00 pm
Location:
Agricultural Sciences Building, Room 315
University of Hawaii at Manoa
1955 East-West Rd. #218
Honolulu, HI 96822
Materials: tropical maize protoplasts and plants
Hands-on experiments and activities:
CRISPR Basics: (History of gene editing, principles, applications)
Overview of our experiment for the workshop (why/significance)
CRISPR How To: (CRISPR construct, talk about each component, go into gRNA design)
gRNA design on ZmCCT10
Tuesday
Protoplast Isolation
Protoplast Transfection with pKL2013
Wednesday
Lecture on cloning and our example
Confocal microscopy to check transfection efficiency
Ligation reaction with T4 DNA Ligase
• Clone gRNA into pKL2020
• Direct plate
Thursday
Check for E. coli growth on plates from transformation
ddPCR lesson
ddPCR on DNA from pKL2013 transgenic plants
Friday
Sequence analyses:
Wrap up and summary
2444 Dole Street
Honolulu, HI 96822