Streaking for Isolation (m140.4.1) - Handouts pg. 52

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Bacterial Growth

Bacteria grow by binary fission and many medically important bacteria will divide once every 20 - 30 minutes under ideal growth conditions. The time it takes for a cell to go from one division to the next is called the GENERATION TIME. This can be determined in culture by either counting bacteria or measuring the optical density of a broth culture and determining the time interval needed for doubling the population. Because of this, the generation time is often called the DOUBLING TIME.

For a complete discussion of bacterial growth see the handouts pgs. 52 (m140.4.1) and Cells alive page on bacterial growth.
 
 

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Handwashing Experiment:

The Effect of Handwashing on Skin Flora (m140.4.2) - Handouts pg. 55

The Handwashing Experiment (m140.4.3) - Handouts pg. 58

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The Fungi 

Fungus Experiments (m140.4.4) - Handouts pg. 61

The Fungi of Medical and Economic Importance (m140.4.5) - Handouts pg. 62

An Overview of the Fungi - An excellent survey taken from "The Tree of Life." You can visit "The Tree of Life" at: ( http://phylogeny.arizona.edu/tree/phylogeny.html )

TORTORA ET.AL.:

• Chapter 6, read about “Obtaining pure cultures” on pg. 162, and please pay attention to fig. 6.11;
• Chapter 14, read about “Pathology, Infection and Disease” and “Normal Microbiota”; also read about “Healthcare-Associated Infections and Emerging Infections” and note “Droplet transmission” in fig. 14.6;
• Chapter. 21: Read about skin structure and look over Staph and Strep infections;
• Chapter 12, read about “Fungi”

LEBOFFE and PIERCE:

• Streak Plate Method of Isolation: pg. 5-6;
• Fungi: pgs. 178-192.

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