The
goal of using a microscope is to obtain
increased magnification, resolution,
and contrast of a specimen.
Magnification depends on the choice of
optics, in this case, the oculars and
objectives.
Resolution
and contrast, however, are largely
dependent on specimen illumination.
Optical illumination for most specimens
is called Kohler Illumination.
Once
a given selection of optics is in
place, Kohler illumination cannot
affect magnification, but it does
affect resolution and
contrast.
It
is easiet to establish Kohler
illumination initially with lower
magnification objectives, then to touch
up alignment while moving to higher
magnifications.
Click
here
to learn how to adjust your microscope
for optimal Kohler
illumination.
