Materials and methods
Specimens of Vitex rotundifolia(L.) were collected from the campus of the University of Hawaii. The leaves, petioles and young stems, and old stems were cut into small pieces were placed separately into a fixative antifreeze solution that contained 10% Dimethylsulfoxide, 4% Formaldehyde, 1% Tween 20 and 0.05M sodium Cacodylate. The pH was set at 7.5. The samples were stored in refrigerator for approximately 2 weeks. Specimens were mounted on stubs in a freezing microtome and sectioned at 50--40 microns. The unstained specimens were mounted with freezing support medium and viewed with microscope. For those that stained with Toluidine Blue (0.05%), Phloroglucinol (saturated solution in 20% HCl), and IKI, the freezing support medium was diluted with water and removed by blotting before any staining solution was applied to the sample.
Specimens were viewed and photographed with brightfield photomicroscope. The pictures were digitized with a Hewlett Packard PhotoSmart scanner at 1200 dots/inch. The pictures were modified with Adobe Photo Delux. Web pages were created with FrontPage 98.