RESULTS:

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Leaf

There are multicellular trichomes present at both the abaxial and abaxial side of the leaves of Vitex rotundifolia (L.).  The amounts of trichomes in  the abaxial side are much denser.  The epidermis in both side of the leaf is one cell layer thick.  One or two layers of collenchyma present just beneath the epidermis.  In the palisade mesophyll region, multilayers of cholorenchyma cells extends to the abaxial layer of the leaf, but they are less dense compare to the adaxial side.  Some air crypts could be observed.  Vascular tissues such as xylem and phloem are present inside the leaf between the chlorenchyma cells.  They are small vascular bundles that located close to the abaxial side of the leaf.

cross section of V. rotundifolia leaf at 50x showing trichomes, epidermis, and palisade.	cross section of V. rotundifolia leaf at 63x showing plaisade, trichomes, and a small vascular bundle between the chlorenchyma cells.

There is a large vascular bundle present in the midrib.  Phloem presents at both the top and bottom of  the xylem.   Vessel members can be seen.  In the midrib region, isodiametric parenchyma cells surround the vascular bundle.  There is no chlorenchyma cells present in this region.

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