ABSTRACT
The anatomy of Vitex rotundifolia (L.) leaves , petioles, young stems, and old stems were determined. This plant has been known for its medical purpose. Plant specimens were collected from the University of Hawaii, Oahu, Hawaii. The specimens were placed in an antifreeze fixative and sectioned with a freezing microtome. The samples stained with Toluidine blue, IKI, and Phloroglucinol and photographed in bright field with photomicroscope. The observation reveal that the plants have several xeromorphic adaptations. It has multicellular trichomes on the adaxial and abaxial side of the leaf, especially abundant in the abaxial epidermis. There are several layers of palisade chlorenchyma. There is one vascular bundle presents in the midrib and couple small vascular bundles present between the chlorenchyma. Both petiole and young stems contain trichomes in their epidermis. Lignified of vascular tissues were observed in the progressing toward secondary growth. The vascular tissues, xylem, phloem, and fibers outline the squared shape of the stem. In the center, the pith of the stem, a lot of starch grains parenchyma cells that function in the storage of nutrients.