Quiz VIII

1. How does temperature affect bacterial growth?

• How does temperature affect enzymes?

2. How are organisms classified according to their temperature requirements?

3. What is Thermus aquaticus?

• How is this organism used in modern molecular biology?
• What is the Polymerase Chain Reaction?

4. How are organisms classified according to their Oxygen requirements?

5. What is catalase?

• How does it protect cells?
• Why would cells have it?
• How does one do the catalase test in the lab?

6. How is Oxygen manipulated in the lab?

• How do you grow capnophilic organisms?
• How does an anaerobe jar work?
• What is thioglycollate used for?

7. Describe the diseases (how do you catch the disease and what are the symptoms) caused by the following organisms:

• Clostridium tetani
• Clostridium botulinum
• Clostridium perfringes
• Clostridium difficile
• Streptococcus pyogenes (group A beta hemolytic Streptococcus)
• Streptococcus faecalis (Enterococcus faecalis; Group D streptococcus)
• Streptococcus pneumoniae (note that there has been a marked increase in antibiotic resistance with this organism recently)
• Streptococcus agalactae (group B streptococcus)
• Check out this "4 8 Hours" story on CBS

• What do the different forms of hemolysis look like?

9. Describe bile esculin agar.

• What is esculin?
• Why would an organism want to break it down?
• How do we know if an organism has broken it down?
• What is bile?
• Which types of organisms would you expect to be tolerant to bile?

10. How can you tell if something is growing in your salt broth?

• What were the results of your salt broth experiment?
• Which organism was the most halophilic?
• Which organism was the most sensitive to the salt?