Kapi‘olani College -- University of Hawai‘i
John M. Berestecky, Ph.D.
Office: Kokio 105;
Rosemarie McElhaney, Ph.D.
Office: Kokio 204
Teena Michael, Ph.D.
Office: Kokio 204
Matt Tuthill, Ph.D.
Office: Iliahi 222
LABORATORY COORDINATOR AND TECHNICIAN:
Colleen Allen, M.S.
Office: Kokio 108C;
Sections Offered - Fall 2012
MEETING TIMES in Kokio 106:
31732 M,W 9:00 – 10:45 -- Berestecky
31733 M,W 11:00 – 12:45 -- Michael
31734 M,W 13:30 – 15:15 – Michael
31737 T, Th 10:00 – 11:45 -- Garcia
31738 T,Th 18:45– 20:10 -- McElheney
TEXTBOOKS and RESOURCES:
(1.) These Web Pages - The material you find here on the web will be the primary laboratory manual for this course. You may call the material up and print it for class or you may use the laboratory computers to access this material.
All experiments, handouts and study guides can be found on the Web and it is important that you view this material. All schedule revisions and changes to the experiments and laboratory exercises will be announced on the Web site. The WWW addresses are:
(2.) Microbiology: An Introduction, Eleventh EditionTORTORA, Funke and Case
The Benjamin/Cummings Publishing Co., Inc. 2013
(3.) A Photographic Atlas for the Microbiology Laboratory, Fourth EditionLEBOFFE and PIERCE
Morton Publishing Company, 2011
(4.) Microbiology 140 Handouts - These are available for purchase at the bookstore. All of the materials in these handouts are also available on the web. In fact, the web is a better source of this information.MEETING TIMES in Kokio 106:
MICR 140 covers the fundamental laboratory aspects of microbiology with a public health and medical emphasis.
Upon successful completion of MICR 140, the student should be able to:
· Use the metric system and scientific notation.
· Use and properly care for the compound microscope, including the oil immersion lens.
· Prepare, examine and accurately interpret various stained slide specimens including gram stained, capsule stained, endospore stained and flagella stained specimens.
· Understand and properly execute the aseptic transfer of bacterial cultures.
· Use sterile pipettes aseptically and accurately.
· Demonstrate the ubiquity of microbes as part of our normal flora and as present in the environment.
· Understand and demonstrate the principles and the techniques which are used to control microorganisms such as antibiotics, food preservatives and the chemical and physical disinfecting and sterilizing agents.
· Enumerate the bacteria in food and water samples and mathematically predict the growth characteristics of these bacteria.
· Understand and demonstrate the effect of different personal hygiene practices on our normal flora and on pathogens.
· Demonstrate and understand the various nutritional requirements and characteristics of medically important bacteria.
· Demonstrate the ability to isolate, in pure culture, and to identify common human commensal bacteria.
· Demonstrate the principles involved in the transmission of pathogenic organisms by the common routes.
· Demonstrate the thinking skills needed to critically observe, measure, evaluate and interpret experimental data and the creativity needed to formulate hypotheses to explain the data.