MICR 431L - Microbial Physiology Lab
Dates:
Tuesdays and Thursdays (1:20 – 5:20 pm)
Location: Snyder 203, Snyder 208 and Snyder 111
TAs: Jimmy Saw and Jing Liu
Phone: 956-6945
E-mail: jimmy AT hawaii.edu, jingliu AT hawaii.edu
Office Location: Snyder 111
Office Hours: By Appointment
Last Updated: 4/28/05 3:04 PM
Notice: Powerpoint lecture is posted and so is the guideline for formal paper. Please read carefully to see what you need to include in the paper.
| Dates | Title | |
| T | 11-Jan | Introduction and overview |
| R | 13-Jan | Signal Transduction overview, experimental design, group assignment |
| T | 18-Jan | Primer design and molecular biology lecture, PCR, purify PCR product |
| R | 20-Jan | EcoRI digestion, geneclean, ligation |
| F | 21-Jan | 1st PCR, purify (done by TA) |
| T | 25-Jan | 2nd PCR, TOPO cloning, transformation, discuss Nature paper |
| W | 26-Jan | Pick up colonies (pm) (done by TA) |
| R | 27-Jan | Plasmid isolation, mini-digest |
| T | 1-Feb | EcoRI mutagenesis PCR, DpnI digestion, transformation |
| W | 2-Feb | Pick up colonies (done by TA) |
| R | 3-Feb | Plasmid isolation, (both pET and pTrc), mini digest, discuss PNAS paper |
| T | 8-Feb | Preparative digest with pET vector and pTrc, Geneclean, ligation |
| W | 9-Feb | Transformation (am), pick up colonies (pm) (done by TA) |
| R | 10-Feb | Plasmid isolation, mini-digest |
| T | 15-Feb | Transform pET into pLysS cells + Quiz1 |
| W | 16-Feb | pick up colonies (pm) (done by TA) |
| R | 17-Feb | Small-scale induction, protein sample prep |
| T | 22-Feb | SDS-PAGE |
| W | 23-Feb | Large-scale induction (done by TA) |
| R | 24-Feb | Protein purification 1 - group 1 and 2 |
| T | 1-Mar | Protein purification 2 - group 3 and 4 |
| R | 3-Mar | SDS-PAGE |
| T | 8-Mar | Immunoblot (transfer), discuss Protein Expression paper |
| R | 10-Mar | Immunoblot (detection), discuss Aerotaxis paper |
| T | 15-Mar | Transform pTrc99A into deletion strain + Quiz2, Aerotaxis and motion analysis lecture |
| W | 16-Mar | pick up colonies, swarming plates (done by TA) |
| R | 17-Mar | Motion analysis 1 - group 1 take data, calculate, plot graph |
| M | 28-Mar | pick up colonies, swarming plates (done by TA) |
| T | 29-Mar | Motion analysis 2 - group 2 take data, calculate, plot graph |
| W | 30-Mar | pick up colonies, swarming plates (done by TA) |
| R | 31-Mar | Motion analysis 3 - group 3 take data, calculate, plot graph |
| M | 4-Apr | pick up colonies, swarming plates (done by TA) |
| T | 5-Apr | Motion analysis 4 - group 4 take data, calculate, plot graph |
| R | 7-Apr | Genome Sequencing, assembly and annotation Lecture |
| T | 12-Apr | Annotation assignment (use computer at Keller309 - 2 people per computer) |
| R | 14-Apr | Annotation and data-mining (on your own or at Keller 309) |
| T | 19-Apr | Visualization demo - using stereo 3D projector |
| R | 21-Apr | Student Presentations |
| T | 26-Apr | Student Presentations |
| R | 28-Apr | Review Session |
| T | 3-May | Final Exam – Cumulative |
| R | 5-May | Reports Due |
Reading Materials
Links to other websites
Cool bacterial motility movies - swimming, twitching, swarming etc