First Model

Model proposed to explain lower copy number of pNEW.
In the case of pNEW (the FullTRP1 Circle) the presence of the full promoter promotes higher level of expression. The higher levels of transcripts products may be intervening with pABF1 (ARS Binding Factor 1) at the B3 domain of ARS1, disrupting the proper chromatin structure require for replication which pABF1 is thought to preserve. ORC = Origin Recognition Complex.