Hawaii Chocolate Microbes Angela Kaaihue Yeasts isolated from cocoa fermentations from various countries


Brazil (Schwan, Wheals, 04)	Ghana	Malyasia	Belize	Unknown country 
Accept (Rohan et al. 1963)

Candida bombi, Candida pelliculosa, Candida rugopelliculosa, Candida rugosa,
Kloeckera apiculata, Kluyveromyces maarxianus, Kluyveromyces thermotolerans, Lodderomyces elongisporus, Pichica fermentans, S. cerevisiae  
var. Chevalieri, Saccharomyces cerevisiae, Torulaspora pretoriensis	Candida spp., Hansenula spp., Kloeckera spp., Pichia spp.,  
Saccharomyces spp., Saccharomycopsis spp., Schizosaccharomyces, spp., Torulopsis spp.	Candida spp., Debaryomyces spp., Hanseniaspora spp.,  
Hansenula spp., Kloeckera spp., Rhodotorula spp., Saccharomyces spp., Torulopsis spp	Brettanomyces clausenii., Candida spp., C. boidinii,  
C. cacoai, C. guilliermondii, C. intermedia, C. krusei, C. reukaufii, Kloeckera apis, Pichia membranafaciens, Saccharomyces cerevisiae,  
Saccharomyces chevalieri, Saccharomycopsis spp., Schizosaccharomyces malidevorans, Schizosaccharomyces spp. 	Sacchaomyces chevalieri,  
Kluyveromyces fragilis, Torulopsis Candida, Candida norvengensis, Kluyveromyces marxianus, Saccharomyces Cerevisiae, Kloeckera sp., Candida  
kursei/incompicua
Candida spp., 
Pichia fermentas, 
Kloeckera japónica, 

Hawaii	Trinidad			
Chevalieri, Saccharomyces				





Lactic acid bacterias isolated from cocoa fermentations from various countries

Brazil (Schwan, Wheals, 04)	Ghana	Malyasia	Belize	Unknown country 
Accept (Rohan et al. 1963)

Lactobacillus, Acidophilus, Lb. Brevis, Lb. casei, Lb. Delbrueckii, Lb. fermentum, Lb. Lactic, Lb. Plantarum Lactococcus lactis, Leuconostoc  
mesenteroides, Pediococcus acidilactici, Pl dextrinicus	Lb. collinoides, Lb. fermentum, Lb. mali, Lb., Plantarum	Lb. collinoides, Lb.  
plantarum	Lb. brevis, Lb. buchneri, Lb. casei, Lb. Casei, pseudoplantarum, Lb. cellobiosus, Lb. delbrueckii, Lb. fermentum, Lb.  
fructivorans, Lb. gasseri, Lb. kandleri, Lb. plantarum, Leuconostoc mesenteroides, Ln. oenos, Ln. paramesenteroides	Lb. lactic, Lb  
plantarum, Lb. acidophilus, Lb. bacillaceae, Lb. parabuchneri Peiococcus pentosaceus, 


Hawaii	Trinidad			
	Streptococcus Lactis (Ostovar, K. Keeny, G.)
			

 
List of identified Acetic Acid bacteria isolated from various countries 

Brazil (Schwan, Wheals, 04)	Ghana	Malyasia	Belize	Unknown country 


Acetobacter aceti subsp. Liquefaciens, A. pasteurianus, A. peroxydans, Gluconobacter oxydans Subs. Suboxydans	Acetobacter ascendens, A.  
rancens, A. xylinum, Glucononbacter oxydans	Acetobacter lovaniensis, A. rancens, A. xylinum, Gluconobacter oxydans	Acetobacter spp.,  
Bluconobacter oxydans	Acetobacter acetum Bkycibibacter ixtdabsm spp. Suboxydans, mucor pusillus, Asperillus fumigatus sp.
         

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Theobroma Cacao- Problems in Fermenting Cacao

 
Theobroma Cacao L.
Problems in Fermenting Cacao

PROJECT BACKGROUND: Fermenting Theobroma Cacao funded by University of Hawaii at Manoa, Department of Molecular Bioscience and Bioengineering. Principal Investigator: Dr. Loren Gautz, PhD. Angela Kaaihue, Candidate for Master's Degree, 08.

Extensive research is being conducted on growing cacao (Theobroma Cacao L.) and improving the quality of chocolate production as a possible crop to make Hawaii's economy thrive and be sustainable. (Cacao and cocoa are used interchangeable in the available literature. The Hawaii industry has adopted the use of cacao for this crop.) In the past, sugar and pineapple were Hawaii's main agricultural crops; however, worldwide competition has made these industries no longer economically sustainable.  Currently, Hawaii is in search of sustainable agricultural crops to occupy fallowed fields and help sustain Hawaii's economy.

Particularly, Hawaii’s cacao growers need bean fermentation technology to make cacao a viable agricultural crop contributing to a sustainable economy.  The production of chocolate from cacao beans requires extensive processing. After pods are harvested from the trees, they are opened and the beans are removed. The beans are covered with sugary mucilage that can be fermented to support conditions that develop the chocolate flavor. Removal of the mucilage is necessary for drying and storing the beans. The raw bean must be fermented to bring out the chocolate flavor that everyone is familiar with. Even though the climate of Hawaii is very good for growing cacao trees it is at the extreme north of the tropical cacao production area. The fermentation technology currently used in the cacao industry has not worked well for Hawaii growers.

 

 

1-Ferment Cacao Beans- The "Green Way"

2-List of Factors which contribute to Reliable and Favorable Quality Chocolate

3- Defined Microbial Cocktail Inocculum (DMCI), fermentation process

4-Identified strains of microbes used in fermentation processes

5-Safety and Health Precations in fermenting cacao beans

6-The benefits of using biotechnological techniques in farming and fermenting processes of theobroma cacao

8-Problems and difficulties that occur in fermenting processes.

9-Market analysis, productions costs, and consumer demands.

10-Literature Research and Other Readings



Current research and data collection are currently in progress. Any further information is classified confidential and may not be considered public. Research at the University of Hawaii at Manoa, Molecular Biosciences and Bioengineering Department. This project is headed by Dr. Loren Gautz, Ph.D.,and Angela Ka'aihue, Master Degree Candidate 2008. Feel free to contact us for further information or questions.