Microsatellites for Ecologists:

Non-Radioactive Isolation

and

Amplification Protocols for microsatellite markers

Rob Toonen

Hawaii Institute of Marine Biology

SOEST, University of Hawaii, Manoa,

PO Box 1346 Kaneohe, HI 96744

formerly:

University of California, Davis

Section of Evolution and Ecology,

Center for Population Biology

Davis, CA 95616

Copyright & Fair Use

This document is protected by the copyright laws of the United States of America. No portion of this document may be reproduced without acknowledgment of the author; the title page and running header constitute appropriate acknowledgment. I hereby grant individuals and organizations permission to reproduce up to 10 (ten) copies of this document (or portions of this document) per version. My permission to produce more than 10 copies of this document must be obtained, prior to such reproduction. No portion of this document may be reproduced or distributed for profit.

This protocol was developed in order to assist non-specialists in the development of “low-tech” microsatellite facilities. If you use any of the information herein, please acknowledge this protocol as:

Toonen, Robert J. Microsatellites for Ecologists: Non-Radioactive Isolation and Amplification Protocols for microsatellite markers, Unpublished manuscript, available from the author or via anonymous FTP from http:/biogeek.ucdavis.edu/Msats/ or http://www2.hawaii.edu/~toonen/files/MsatsV1.pdf

Thanks, and if you have any questions or problems with the techniques/protocols listed herein, or just want to make some suggestions, please feel free to drop me a note (remove the underscores to make this Email work: toonen_@_hawaii.edu).

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Adobe Acrobat Format : MsatsV1.pdf (719kb)