Lecture 1: Welcome to Molecular Biology - Malacinski Chapter 1
Lecture 5: Macromolecule Complexes
Lecture 6: The Genetic Material
Laboratory Exercises
Exercise 4 - Lambda and pBR322 Digestions
MarciaDigestion of Isolated Genomic and Plasmid DNA:
Lana and Shana distilled water
Ling Hua and Marcia distilled water
Ling Hua and Marcia milliq water
SalI Digest - Numerous Attempts
Chris and Grace, first attempt -- plenty of digested genomic, no plasmid!
Chris, second attempt -- plenty of digested genomic, plasmid didn't digest
Chris, third attempt -- everything digested, gel ran with Ling Hua
Lana and Shana, first attempt -- plenty of genomic, no plasmid!!
Lana and Shana, second attempt -- plenty of digested genomic and digested plasmid!
Ling Hua, first attempt -- plenty of digested genomic, no plasmid!!
Ling Hua, second attempt -- YIKES!!! Wha' dat?!
Ligation of digested genomic DNA into pGEM
Transformed E. coli Strain Analysis
1. Restriction Digests
2. PCR
GRAND LAB REPORT -- Due Dec. 14, 2006!