Molecular Biology

Lecture 1: Welcome to Molecular Biology - Malacinski Chapter 1

Lecture 2: Macromolecules

Lecture 3: Nucleic Acids

Lecture 4: Proteins

Lecture 5: Macromolecule Complexes

Lecture 6: The Genetic Material

Lecture 7: DNA Replication

Lecture 8: Transcription

Lecture 9: Translation

 

Laboratory Exercises

Exercise 4 - Lambda and pBR322 Digestions

Lana

Shana

Linghua

Grace

Chris

Marcia

Digestion of Isolated Genomic and Plasmid DNA:

Chris and Grace

Lana and Shana distilled water

Lana and Shana milliq water

Ling Hua and Marcia distilled water

Ling Hua and Marcia milliq water

SalI Digest - Numerous Attempts

Chris and Grace, first attempt -- plenty of digested genomic, no plasmid!

Chris, second attempt -- plenty of digested genomic, plasmid didn't digest

Chris, third attempt -- everything digested, gel ran with Ling Hua

Lana and Shana, first attempt -- plenty of genomic, no plasmid!!

Lana and Shana, second attempt -- plenty of digested genomic and digested plasmid!

Ling Hua, first attempt -- plenty of digested genomic, no plasmid!!

Ling Hua, second attempt -- YIKES!!! Wha' dat?!

Ling Hua, third attempt -- plenty of digested genomic and digested plasmid. (Chris is on this gel too.)

Ligation of digested genomic DNA into pGEM

Chris and Grace Ligation

Ling Hua Ligation

Shana and Lana Ligation

Transformed E. coli Strain Analysis

1. Restriction Digests

Chris

Ling Hua

Lana and Shana

2. PCR

Chris

Lana and Shana

Ling Hua

GRAND LAB REPORT -- Due Dec. 14, 2006!