MICRO 130 STUDY GUIDE

SAMPLE SECOND EXAM

This should give you a pretty good idea of the types of questions I like to pose. Hopefully you will discuss and analyze them with other students in the class. If you're really stuck in trying to come up with an answer, call me (734-9123) or e-mail me (johnb@hawaii.edu) and I will get back to you.

Some questions refer to figures which unfortunately haven't been placed into this document yet. In most cases I believe that the context of the question will inform you about the facts and ideas you could be tested on. If you do want to see the figure, the exam on reserve in the library has the diagrams - but the questions are not in the same order as they are here.

MICROBIOLOGY 130
NAME____________________________________________
SECOND MIDTERM - October 26,1994

1. A child has type AB blood. Which of the following statements is incorrect?

2. Which of the following is mismatched 3. Which of the following is a mutagen found in moldy peanuts 4. What is false about this diagram?  5. The DNA probe, 5' G G C T T A, will hybridize with which single stranded DNA? 6. Which of the following organisms would probably be most sensitive to penicillin? 7. Deleting the G in the following gene sequence:

T A C G T T A C A

8. A chemical agent that can remove pathogens from table tops is 9. Ten Staphylococcus aureus cells fell from your nose into the macaroni salad you were making for a picnic. Given a generation time of 15 minutes, how many bacteria would you have after 2 hours? 10. If you knew the sequence of nucleotides within a gene which of the following could you predict MOST ACCURATELY? 11. What is false about this diagram?  12. Many simple eucaryotic genes can be transcribed and translated by procaryotes like E. coli. However some eucaryotic genes cannot be expressed by procaryotes. Which of the following might be a reason for this failure? 13. Use the following information to answer the question below:
 
Codon on mRNA and the corresponding amino acid     
AUG - methionine  UAA - nonsense  UUA - leucine 
GCA - alanine  AAG - lysine  GUU - valine 
AAU - asparagine  UGC - cysteine  UCG, UCU - serine 
 

What is the sequence of amino acids coded for by the following DNA
sequence: 3' T A C A A T T T C A G C

14. Which of the following is a product of translation 15. DNA fingerprinting 16. Electrophoresis 17. Which of the following could be used to sterilize plastic petri dishes in a plastic wrapper? 18. Which of the following will influence how long it takes to eliminate bacteria from an object? 19. A nuclease 20. Which of the following statements is true about alleles 21. What is a promotor? 22. What is true about mutation? 23. What is true about the Lac Operon? 24. The catabolic process in which pyruvic acid is converted into lactic acid: 25. Making new copies of DNA from old copies of DNA is known as: 26. The code for the synthesis of enzymes is originally stored in: 27. The catabolic process in which pyruvate is converted into NADH2, FADH2, GTP and CO2 28. An organism that uses carbohydrate for energy and carbon 29. When one molecule of NADH2 passes through the electron transport chain, how many molecules of ATP are produced? 30. Which statement is false about this diagram?  31. What is false about this graph?  32. Which of the following procedures will not kill endospores? 33. High salt and/or high sugar concentrations are used to preserve foods like salt plum, salted fish and fruit jams and jellies. Salt and sugar will preserve these foods because: 34. Put the following into the correct sequence for protein synthesis: 1.) translation 2.) RNA polymerase transcribes the gene 3.) polypeptide is released 4.) mRNA binds to the ribosome 35. Oxygen is poisonous for: 36. To sterilize an object means the destruction or removal of: 37. A faculative anaerobe: 38. Differences in the procaryotic and eucaryotic ribosomes allow some antibiotics to selectively inhibit the synthesis of bacterial: 39. Which group of organisms are most likely to grow in cold seawater? 40. Nucleic acid hybridization is based on which property of DNA? 41. When applied to wounded tissue, hydrogen peroxide: 42. Antibiotics like chloramphenicol, erythromycin and tetracycline inhibit translation in procaryotes but not in eucaryotes. The most likely target of these antibiotics would be the: 43. Which of these chemicals is the least effective antimicrobial agent? 44. A liquid that has been "sterilized" by filtration may still contain: 45. Antibiotics that are rapidly excreted from the body in the urine are probably: 46. Silver nitrate is used 47. Many antiviral chemotherapeutic agents are similar to AZT in that they 48. Which of the following statements is false about oxygen and bacterial cells 49. Semi-conservative replication means that each new genome will have 50. Takes free-floating nucleotides and joins them together to make a strand of DNA RETURN TO THE KCC MICROBIOLOGY HOME PAGE

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